WO2009113795A3 - Vector and method for expressing avian influenza virus neuraminidase n1 in e. coli, method of using the vector, and neuraminidase inhibitors - Google Patents

Vector and method for expressing avian influenza virus neuraminidase n1 in e. coli, method of using the vector, and neuraminidase inhibitors Download PDF

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Publication number
WO2009113795A3
WO2009113795A3 PCT/KR2009/001184 KR2009001184W WO2009113795A3 WO 2009113795 A3 WO2009113795 A3 WO 2009113795A3 KR 2009001184 W KR2009001184 W KR 2009001184W WO 2009113795 A3 WO2009113795 A3 WO 2009113795A3
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WO
WIPO (PCT)
Prior art keywords
vector
neuraminidase
coli
avian influenza
influenza virus
Prior art date
Application number
PCT/KR2009/001184
Other languages
French (fr)
Other versions
WO2009113795A2 (en
Inventor
Doman Kim
Young-Min Kim
Do-Won Kim
Hurng-Chun Lee
Ying-Ta Wu
Vincent Breton
Original Assignee
Industry Foundation Of Chonnam National University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Industry Foundation Of Chonnam National University filed Critical Industry Foundation Of Chonnam National University
Publication of WO2009113795A2 publication Critical patent/WO2009113795A2/en
Publication of WO2009113795A3 publication Critical patent/WO2009113795A3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01018Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/04Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Abstract

Disclosed are a vector and method for expressing neuraminidase Nl of avian influenza (AI) virus in E. coli, a method of using the vector to screen an inhibitor of neuraminidase Nl, and a novel neuraminidase Nl inhibitor screened out by the method.
PCT/KR2009/001184 2008-03-10 2009-03-10 Vector and method for expressing avian influenza virus neuraminidase n1 in e. coli, method of using the vector, and neuraminidase inhibitors WO2009113795A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0021970 2008-03-10
KR1020080021970A KR20090096890A (en) 2008-03-10 2008-03-10 Vectors and processes for expression of neuraminidase n1 of avian influenza virus in e. coli, methods of using the same, and agents for inhibiting activities of the neuraminidase

Publications (2)

Publication Number Publication Date
WO2009113795A2 WO2009113795A2 (en) 2009-09-17
WO2009113795A3 true WO2009113795A3 (en) 2010-02-25

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/001184 WO2009113795A2 (en) 2008-03-10 2009-03-10 Vector and method for expressing avian influenza virus neuraminidase n1 in e. coli, method of using the vector, and neuraminidase inhibitors

Country Status (2)

Country Link
KR (1) KR20090096890A (en)
WO (1) WO2009113795A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8575197B2 (en) 2008-09-25 2013-11-05 Shionogi & Co., Ltd. Pyrolinone derivative and pharmaceutical composition comprising the same

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102822343B (en) * 2009-12-28 2017-10-20 赛诺菲疫苗技术公司 The production of heterologous polypeptide in microalgae, the extracellular body of microalgae, composition and its production and use
CN103951602B (en) * 2014-03-19 2016-06-15 河南理工大学 There is the synthetic method of pyrroles's thiosemicarbazones copper of anti-tumor activity, nickel complex
US20180303807A1 (en) * 2015-10-01 2018-10-25 John Katzenellenbogen Androgen receptor ligands
CN105646495A (en) * 2016-01-06 2016-06-08 中山大学 Pyrrolo[2,3-b]pyrazine derivatives, and preparation method and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4537769A (en) * 1982-04-06 1985-08-27 American Cyanamid Company Stabilization of influenza virus vaccine

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4537769A (en) * 1982-04-06 1985-08-27 American Cyanamid Company Stabilization of influenza virus vaccine

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
QI-SHI DU ET AL.: "Analogue inhibitors by modifying oseltamivir based on the crystal neuraminidase structure for treating drug-resistant H5N1 virus", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 362, no. 2, 13 August 2007 (2007-08-13), pages 525 - 531 *
SONTANA SIRITANTIKORN ET AL.: "Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus(H5Nl) recombinant proteins", JOURNAL OF IMMUNE BASED THERAPIES AND VACCINES, vol. 5, no. 1, 3 October 2007 (2007-10-03), pages 1 - 5 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8575197B2 (en) 2008-09-25 2013-11-05 Shionogi & Co., Ltd. Pyrolinone derivative and pharmaceutical composition comprising the same

Also Published As

Publication number Publication date
KR20090096890A (en) 2009-09-15
WO2009113795A2 (en) 2009-09-17

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